Gene expression in primary neurons upon knockdown of Diras2

Published: 30-11-2018| Version 1 | DOI: 10.17632/rtsx7zpt4w.1
Contributors:
Lena Grünewald,
Andreas G Chiocchetti,
Heike Weber,
Claus-Jürgen Scholz,
Christoph Schartner,
Florian Freudenberg,
Andreas Reif

Description

The expression of 88 target genes and three reference genes (Pgk1, Rpl13a and Sdha) was analyzed in singlets using a RealTime ready Custom Panel 384 – 96+ with Light Cycler 480 Probes Master on a LightCycler480 (Roche) using the following conditions: i) 95 °C for 10 min, ii) 45 cycles of 95 °C for 10 s, 60 °C for 30 s, 72 °C for 1 s co-terminated by a plate read. AAV2 = cells treated with AAV-U6-Diras2_2-hSyn::mCherry.3xFLAG-WPRE AAV4 = cells treated with AAV-U6-Diras2_1-hSyn::mCherry.3xFLAG-WPRE Scr = cells treated with AAV-U6-Scrambled-hSyn::mCherry.3xFLAG-WPRE Unb = untreated cells

Files

Steps to reproduce

.ixo Files are raw data files from Roch LighCycler480 and need to be imported into the LightCycler480 software