Gene expression in primary neurons upon knockdown of Diras2

Published: 30 November 2018| Version 1 | DOI: 10.17632/rtsx7zpt4w.1
Contributors:
Lena Grünewald,
Andreas G Chiocchetti,
Heike Weber,
Claus-Jürgen Scholz,
Christoph Schartner,
Florian Freudenberg,
Andreas Reif

Description

The expression of 88 target genes and three reference genes (Pgk1, Rpl13a and Sdha) was analyzed in singlets using a RealTime ready Custom Panel 384 – 96+ with Light Cycler 480 Probes Master on a LightCycler480 (Roche) using the following conditions: i) 95 °C for 10 min, ii) 45 cycles of 95 °C for 10 s, 60 °C for 30 s, 72 °C for 1 s co-terminated by a plate read. AAV2 = cells treated with AAV-U6-Diras2_2-hSyn::mCherry.3xFLAG-WPRE AAV4 = cells treated with AAV-U6-Diras2_1-hSyn::mCherry.3xFLAG-WPRE Scr = cells treated with AAV-U6-Scrambled-hSyn::mCherry.3xFLAG-WPRE Unb = untreated cells

Files

Steps to reproduce

.ixo Files are raw data files from Roch LighCycler480 and need to be imported into the LightCycler480 software

Categories

Neuroscience, Biological Psychiatry, Molecular Neuroscience

License