SARS-CoV-2 reactive and neutralizing antibodies discovered by single cell sequencing of plasma cells and mammalian display

Published: 9 December 2021| Version 1 | DOI: 10.17632/rvd7999gk4.1
Roy Alexander Ehling


In this study, we describe a technology pipeline that integrates single-cell antibody repertoire sequencing and mammalian display to interrogate the specificity of plasma cells from 16 convalescent patients. Single-cell sequencing allows us to profile antibody repertoire features and identify expanded clonal lineages. The deposited data covers the 132 selected heavy and light chain paired antibodies extracted from single-cell plasma cell sequencing, as well as the analysis of specificity post NGS of mammalian display with patient breakdown.



Eidgenossische Technische Hochschule Zurich Department of Biosystems Science and Engineering


Molecular Biology, Antibody Engineering, Immunogenetics, High-Throughput Sequencing