SARS-CoV-2 reactive and neutralizing antibodies discovered by single cell sequencing of plasma cells and mammalian display
Published: 9 December 2021| Version 1 | DOI: 10.17632/rvd7999gk4.1
Contributor:
Roy Alexander EhlingDescription
In this study, we describe a technology pipeline that integrates single-cell antibody repertoire sequencing and mammalian display to interrogate the specificity of plasma cells from 16 convalescent patients. Single-cell sequencing allows us to profile antibody repertoire features and identify expanded clonal lineages. The deposited data covers the 132 selected heavy and light chain paired antibodies extracted from single-cell plasma cell sequencing, as well as the analysis of specificity post NGS of mammalian display with patient breakdown.
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Institutions
Eidgenossische Technische Hochschule Zurich Department of Biosystems Science and Engineering
Categories
Molecular Biology, Antibody Engineering, Immunogenetics, High-Throughput Sequencing