Microbial data and processing data of surface water in Xiantao constructed wetland

Published: 07-05-2018| Version 1 | DOI: 10.17632/ry79c7p3g8.1
Shufen Song


surface water samples directly put the sampler under the surface of water for 0.1-0.3 meters. DNA extraction were firstly collected into sterile Polyethylene plastic bottle(volume is at least 1L) using a sterilizing sampling bottle. The collected water samples are then stored in a box filled with sufficient ice to keep the sample temperature below 4°C during transit. When we arrived in the laboratory, we used microporous membrane (0.22 m) to filter water samples, filtered pore size filter membrane, stored in -80°C refrigerator, DNA was extracted with Water DNA Kit kit from OMEGA company, The extracted DNA was amplified using a specific set of bar-code 515F and 909R. Purification was recovered by Beijing Kang for century general DNA purification recovery Kit, and finally sent to the Chinese Academy of Sciences, Chengdu Institute of biology sequenced by using an Illumina Miseq platform DNA. In addition, Determination and analysis of water physical and chemical indexes: surface water (CODCr) was determined by potassium dichromate titration,TN by alkaline potassium persulfate digestion UV spectrophotometry spectrophotometric method, TP using potassium persulfate digestion ammonium molybdate spectrophotometric method, NH4+-N using Nessler's reagent colorimetric method.