Network characterization data assessed in the chicken aortic ring assay using human adipose-derived stem cell secretomes
Description
We present data assessed with the chicken aortic ring assay in the following six Excel files. First, we provide a data set covering the vessel area, maximum outgrowth radius and number of endpoints per aortic ring for the following matrices: egg white, bovine collagen, jellagel and matrigel (DiB_Figure_1_manuscript vessel network complexity). Second, secretome harvested from human adipose-derived stem cells (3 donors) cultivated as single cells, 250 cells/spheroid or 8000 cells/spheroid were applied in the chicken aortic ring assay. We provide data on the spheroid size, the protein concentration of the three secretomes, the weight of the embryos used for these experiments, the circumference of the aortic rings, the migration extent at days 1,4 and 7, the percentage of initial vessels and the number of loops per aortic ring (DiB_Figure_2_manuscript vessel network complexity). Next, the network was characterized at days 1,4 and 7. Here, we provide data on maximum outgrowth radius, maximum initial vessel length, speed mean vessel length and speed maximum vessel length, respectively (DiB_Figure_3_manuscript vessel network complexity). After that, sprouting parameters are presented. Raw data on vessel density, number of branches, mean branch length, relative frequency of branch length, number of junctions and number of junctions per initial vessels are provided (DiB_Figure_4_manuscript vessel network complexity). Finally, the raw data for these parameters for each donor are presented (DiB_Figure_S2_manuscript vessel network complexity); and the calculated angiogenic activity index (AAI) for each parameter is provided (DiB_Table_S1_manuscript vessel network complexity).
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Funding
Swiss National Science Foundation
310030_197578