CD146 regulates growth factor-induced mTORC2 activity independent of the PI3K and mTORC1 pathways. Xu et al

Published: 27 September 2019| Version 2 | DOI: 10.17632/rzc6mt4zww.2
Contributors:
Wenyi Xu,
Huijuan Hua,
Yueh-Ho Chiu,
Guannan Li,
Huihan Zhi,
Zhengquan Yu,
Fazheng Ren,
永挺 罗,
Wei Cui

Description

These files contain all the original immunoblot images for the 7 main figures and 7 supplementary figures in the paper. A separate zip file was created for each figure. The figure panel and a brief description have been labeled for each blots. For immunoblotting, proteins were resolved in 8-10% SDS-polyacrylamide gel by electrophoresis and then transferred to a polyvinylidene difluoride (PVDF) membrane. The membranes were blocked with 5% non-fat milk in Tris-buffered saline with 0.1% Tween-20 for 1 hour, incubated with primary antibodies at 4℃ overnight and then probed with horseradish peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies. The signals are visualised using chemiluminescence imaging system (Amersham Imager 600, GE Healthcare Life Sciences).

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Institutions

Imperial College London, China Agricultural University

Categories

Molecular Biology, Cell Biology, Cell Adhesion Molecules, Mammalian Target of Rapamycin, Receptor Tyrosine Kinase, Growth Factor, Cell Proliferation, Endothelial Cell, Degradation

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