CD146 regulates growth factor-induced mTORC2 activity independent of the PI3K and mTORC1 pathways. Xu et al

Published: 27 September 2019| Version 2 | DOI: 10.17632/rzc6mt4zww.2
Wenyi Xu,
Huijuan Hua,
Yueh-Ho Chiu,
Guannan Li,
Huihan Zhi,
Zhengquan Yu,
Fazheng Ren,
永挺 罗,
Wei Cui


These files contain all the original immunoblot images for the 7 main figures and 7 supplementary figures in the paper. A separate zip file was created for each figure. The figure panel and a brief description have been labeled for each blots. For immunoblotting, proteins were resolved in 8-10% SDS-polyacrylamide gel by electrophoresis and then transferred to a polyvinylidene difluoride (PVDF) membrane. The membranes were blocked with 5% non-fat milk in Tris-buffered saline with 0.1% Tween-20 for 1 hour, incubated with primary antibodies at 4℃ overnight and then probed with horseradish peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies. The signals are visualised using chemiluminescence imaging system (Amersham Imager 600, GE Healthcare Life Sciences).



Imperial College London, China Agricultural University


Molecular Biology, Cell Biology, Cell Adhesion Molecules, Mammalian Target of Rapamycin, Receptor Tyrosine Kinase, Growth Factor, Cell Proliferation, Endothelial Cell, Degradation