Early pregnancy serum exosomes miRNAs gene-targets vary based on pancreatic β-cell function in GDM
Description
Genes regulated by the miRNA set differentially expressed in maternal or placental serum exosomes in women who will develop GDM based on early pregnancy HOMA-% β index status. Differential expression of miRNA profiles among study subgroups using Affymetrix Transcriptome Analysis Console (TAC) Software version 4.0.1 (Affymetrix, Applied Biosystems). The results were preprocessed at RMA+DABG-NONORM-Human-only setting. The t-test, Multiple Testing Corrections, and False Discovery Rate Prediction were used for this purpose. Those genes with an FDR-adjusted p-value less than 0.05 demonstrated at least a 2-fold difference between the control group and the contrast subgroups were considered statistically significant and differentially expressed. Microarray data have been deposited in the Gene Expression Omnibus (GEO) database with accession number GSE243374. Once the DEmiRNAs were identified for each subgroup, target gene prediction for each DEmiRNA identified was performed using TargetScanHuman 8.0 database (20). TargetScanHuman’s cumulative weighted context score (CWCS) of -0.7 or lower was used as a confidence level filter for miRNA targeting. Each CWCS indicates that a microRNA represses a certain mRNA target by at least 38% as compared to the normal baseline level. Once the target genes were identified, gene expression data analysis was performed using ExpressAnalyst (https://www.expressanalyst.ca). Gene expression statistical and functional global enrichment networks using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database were generated for each comparison condition between the control and the contrast subgroups. KEGG 2021 Human Pathway analysis were performed using the Enrichr platform. Table S1. Genes regulated by the miRNA set differentially expressed in maternal serum exosomes in women who will develop GDM with a high HOMA-% β index Table S2. Genes regulated by the miRNA set differentially expressed in maternal serum exosomes in women who will develop GDM with a low HOMA-% β index Table S3. Genes regulated by hsa-miR-6724-5p present in maternal serum exosomes in women who will develop GDM regardless of their HOMA-% β index Table S4. Genes regulated by the miRNA set differentially expressed in placental serum exosomes in women who will develop GDM with a high HOMA-% β index Table S5. Genes regulated by the miRNA set differentially expressed in placental serum exosomes in women who will develop GDM with a low HOMA-% β index Table S6. Genes regulated by hsa-miR-3665 identified in placental serum exosomes in women who will develop GDM regardless of their HOMA-% β index