BioMnoTrpyCD

Published: 26 October 2021| Version 1 | DOI: 10.17632/s4r6xfdhjs.1
Contributors:
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Description

Provided is raw data expressed as Ct values corresponding to gene expression of human chemokines and their receptors analyzed by using RT² Profiler™ PCR Array Human Chemokines & Receptors (GeneGlobe ID - PAHS-022Z, Qiagen) on a CFX Connect Real-Time PCR System (Biorad, Hercules, CA). Samples: Healthy, mildly and severely inflamed tissue biopsies were collected from all patients and preserved in RNAlater. Tissue samples were obtained from 20 patients of Cronh's disease, grouped by their treatment in 4 groups (untreated, adalimumab, ustekinumab and vedolizumab). Each treatment group has 5 patients associated, with 3 samples from each patient corresponding to the tissue's relative degree of inflammation (Healthy tissue, mildly inflamed tissue, severely inflamed tissue). Clinical and analytical characteristics of patients were recorded at inclusion in the study. Disease clinical activity was determined by Crohn’s disease activity index (CDAI)>150 and presence of clinical symptoms of relapse. Disease endoscopic activity was determined by Simple Endoscopic Score for Crohn Disease (SES-CD). All patients were Caucasian of Mediterranean ethnicity and were classified according to the Montreal classification. All included patients received diaries to record symptoms 1 week before inclusion and sample collection, and signed an informed consent to participate in the study. The study was approved by the Ethics Committee of Hospital General Universitario and performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments. All patients gave their informed consent prior to their inclusion in the study.

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Steps to reproduce

Gene expression of human chemokines and their receptors analyzed by using RT² Profiler™ PCR Array Human Chemokines & Receptors (GeneGlobe ID - PAHS-022Z, Qiagen) on a CFX Connect Real-Time PCR System (Biorad, Hercules, CA) according to manufacturer instructions.

Institutions

Universidad Miguel Hernandez de Elche

Categories

Crohn's Disease

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