27-color healthy lung flow cytometry dataset

Description

Dataset belonging to the publication "Optimized full-spectrum flow cytometry panel for deep immunophenotyping of murine lungs" by Baumann et al. Files are raw flow cytometry data for a healthy female murine lung, stained with a 27-color antibody panel (see Figure 4 and Supplementary Table 2 in the paper). The folder ("raw") contains two subfolders, with corresponding reference single stains and lung samples. In the reference sample folder, the files are either beads or cells, corresponding to what we used for data unmixing in the manuscript. Additionally, it contains the single AF signatures used for unmixing (AF 5, 13, and 35). In the samples folder, FS is the fully stained sample. FMO denotes Fluorochrome Minus One controls. For all questions, please refer to the contact linked in the paper.

Steps to reproduce

For the step-by-step protocol, please refer to the manuscript.

Institutions

• Universitat Basel Departement Biomedizin
• Universitatsspital Basel Bereich Chirurgie

Categories

Funders

• Swiss National Science Foundation

  Switzerland
• Arnold U. and Susanne Huggenberger-Bischoff Foundation for Cancer Research
• Peter Bockhoff Foundation
• Fondation Bryn Turner-Samuels
• Pedersen Charity Foundation
• Hedy-Glor-Meyer Foundation
• Freiwillige Akademische Gesellschaft Basel
• Jubiläumsstiftung of SwissLife
• European Research Council

  Belgium

  Grant ID: 694033 STEM-BCPC
• Krebsliga Beider Basel

  Switzerland
• Swiss Cancer League

  Switzerland

  Grant ID: KFS-4414-02-2018
• Swiss Personalized Health Network

  Grant ID: Swiss Personalized Oncology driver project
• Department of Surgery of the University Hospital Basel

Licence