On the Venom Solenopins alkaloids of Fire Ants: Beware a Lonely Queen

Published: 3 October 2018| Version 1 | DOI: 10.17632/s8x82r464b.1
Contributors:
Eduardo Fox,
Li Chen,
XiaoQing Wu

Description

The present dataset includes background information and files relative to the submitted paper " Venom Isosolenopsin A Delivers Rapid Knockdown of Fire Ant Competitors " currently under review in Toxicon. Currently it serves as a repository to files reviewers may wish to see. After publication it shall host all relevant files pertaining the final published version of the manuscript. It includes: - Original GC-MS chromatogram files obtained for natural venoms, calibration standards, synthetic alkaloids. - R script behind the plotted figures and data analyses; R data. - Supplementary videos and isolated images showing venom- and alkaloids- intoxicated ants.

Files

Steps to reproduce

- Venom of fire ants can be extracted an masse as described in Fox et al. (2013). In short living ants are separated from soil by flotation and immersed alive in a mixture of solvents. -Cis- and Trans- alkaloid fractions are obtainable from this extract as described in Shi et al. (2014). In short, the alkaloid-rich extract is washed through a silica column with increasing hexane:acetone ratios. -Living fire ants can be triggered to release a 20-30nl droplet of venom by gently touching the dorsal gasteral surface above their stingers. - Other ants to be tested can be obtained from the field by excavations. Ants can be temporarily anesthetized with either CO2 , cold temperature, or ethers. - Anesthetized ants can be briefly manipulated with fine tweezers to be touched with a 20-30 nl droplet of venom. - Ants can be likewise manipulated to be touched with a fine capillary presenting a 20-30nl droplet of alkaloid extract. - Time elapsed for complete knockout can be measured with a timer from the moment of contact. - Mortality rates after contact are measured by counting the number of dead ants within intervals over a period of 12h. - Data was analysed and plotted with R software.

Institutions

Institute of Zoology Chinese Academy of Sciences, Universidade Federal do Rio de Janeiro Instituto de Biofisica Carlos Chagas Filho, South China Agricultural University

Categories

Ecology, Zoology, Entomology, Science, Chromatography, Chemical Defenses, Chemical Ecology, Insects, Gas Chromatography, Animals, Animal Science, Biogeography, Nicotinic Acid, Alkaloid, Nicotine, Toxins, Poison, Zoo Animal Species, Competition, Biological Invasion, Invasive Species, Delayed Neurotoxicity, Insecticide, Neurotoxin, Venom, Arthropod Venom, Natural Product Insecticide, Ant, Toxinology

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