PUFA overnutrition - Plantaris Muscle Morphometry Paper 2022
Data used for the elaboration of the scientific article refer to body characteristics, plasma profile, skeletal muscle structure, fiber types profile, neuromuscular junction profile, and extracellular matrix composition. The animals used in the present study are offspring of rats that received excessive supplementation with polyunsaturated fatty acids from the omega 3 and 6 families throughout pregnancy and lactation.
Steps to reproduce
Females rats were split into 3 experimental groups: control (CT group), supplemented with fish oil (FO group), or soybean oil (SO group). The FO used was a mixed marine triacylglycerol preparation containing 180 g eicosapentaenoic acid (EPA) and 120 g docosahexaenoic acid (DHA), purchased from Vitamins Sundown Naturals© (Recife, Pernambuco, Brazil). The SO used was composed primarily of triacylglycerol with 50% linoleic acid purchased from Bunge© (Luziânia, Goiás, Brazil). The oils were supplemented at a level of 4 g/kg b.w. per day and were provided as a single bolus using a pipette for 21 days before and during mating, gestation, and lactation periods, making a total of 68 ± 5 days. At weaning (21 days), male offspring were housed in groups of four animals and maintained until the end of the experiment. At 60 days of age, the animals (n = 12 per group) were euthanized by decapitation, blood collected, and plasma obtained by centrifugation. Bodyweight, naso-anal length, and Lee index, body adiposity index were calculated. The plantaris muscle was dissected, the right antimere was wrapped in neutral talc, frozen in liquid nitrogen, and stored in an ultrafreezer for further histomorphological analysis, while the left antimere was fixed and stored in Karnovsky for histochemical analysis of the neuromuscular junctions.