data of RG108
Description
data of RG108 influence fear extinction
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In the RG108 experiment, mice received intraperitoneal injections of RG108 (N-Phthalyl-L-tryptophan 0.2 mg/kg; R8279; Sigma-Aldrich Company, USA). RG108 was dissolved in dimethyl sulfoxide (D2438-5x10ML; Sigma-Aldrich) at a concentration of 10 mg/ml, stored at −20 °C, and diluted 50 times in vehicle for intraperitoneal injection. The intraperitoneal injections were administered 2 hours before the fear extinction experiment. For the CNO experiment, mice were intraperitoneally injected with clozapine N-oxide (CNO) (10 mg/kg; A3317; APExBio Company, USA). CNO was dissolved in dimethyl sulfoxide (317275-100ML; Sigma-Aldrich) at a concentration of 33 mg/ml, stored at – 20℃, and diluted 50 times in vehicle for intraperitoneal injection (the brain slice of CNO experiment were same samples with our previous article [9]). In the methionine (L-methionine, L-M) experiment, mice were intraperitoneally injected twice daily with L-methionine (5.2 mmol/kg, M9625, Sigma-Aldrich, USA) for 7 days and perfused with a PBS solution before each injection, the behavior test commenced 2 hours after the injection.