SSH library biostimulant-Banana and Pseudocercospora fijiensis interaction
The use of biostimulants induce the expression of genes involved in different metabolic pathways, and may be involved in plant response after pathogen infection. A bioassay was designed using banana plants, derived from in vitro propagation. Plants were grown in pots at greenhouse conditions, and inoculated with the pathogen Pseudocercospora fijiensis (2*10^4 conidia/ml), treatments included the application of a biostimulant (30%) and mock (H2O) application. The biostimulant is a liquid solution that was elaborated in greenhouse using anaerobic microorganisms with plant and organics remains. This solution was used as a biostimulant. To identify differentially expressed genes, associated to the plant response and detection of the pathogen, we used the subtractive suppressive hybridization (SSH) strategy. Two separated libraries were generated, (i) biostimulant application directly to the leaves of banana plants (L1), and (ii) application of biostimulant directly to the soil (L2). A total of 176 unique SSH sequences for the library of foliar application (L1) and 116 unique SSH sequences for the soil application (L2) were isolated, and were analyzed using the Blast2Go software. Highlighted results for L1 and L2 genes identified were related to metabolic process (biological process) and binding (molecular function).