Raw data from: Optimization of the Composition of Mesoporous Polymer–Ceramic Nanocomposite Granules for Bone Regeneration

Published: 20 July 2023| Version 1 | DOI: 10.17632/sbsn69r3rg.1
Marta Trzaskowska, Vladyslav Vivcharenko, Wojciech Franus, Tomasz Goryczka, Adrian Barylski, Agata Przekora


The aim of this research was to optimize the concentration of individual components of polymer–ceramic nanocomposite granules (nanofilled polymer composites) for application in orthopedics and maxillofacial surgery to fill small bone defects and stimulate the regeneration process. Two types of granules were made using nanohydroxyapatite (nanoHA) and chitosan-based matrix (agarose/chitosan or curdlan/chitosan), which served as binder for ceramic nanopowder. Different concentrations of the components (nanoHA and curdlan), foaming agent (sodium bicarbonate—NaHCO3), and chitosan solvent (acetic acid—CH3COOH) were tested during the production process. Agarose and chitosan concentrations were fixed to be 5% w/v and 2% w/v, respectively, based on our previous research. Subsequently, the produced granules were subjected to cytotoxicity testing (indirect and direct contact methods), microhardness testing (Young’s modulus evaluation), and microstructure analysis (porosity, specific surface area, and surface roughness) in order to identify the biomaterial with the most favorable properties. This is a dataset related to the recently published article (Molecules 2023, 28, 5238. https://doi.org/10.3390/molecules28135238). Folder: “Live_Dead” contains raw CLSM images of the cells on the granules after live/dead staining. Folder: “MTT” contains the raw results of three repetitions of MTT cytotoxicity assay performed after 24 and 48 hours of culture. The results of MTT test are expressed as OD values. Folder: “SSA” contains Microsoft Excel files with results of the total surface area measurement performed for each sample. Folder: “SEM” contains raw SEM images of tested granules. Folder: “Stereoscopic images” contains raw stereoscopic images of the granules. Folder: “Roughness” contains raw 3D images of granules surface and a Microsoft Excel file (Roughness.csv) contains the results of areal surface roughness of each tested granule expressed as Sa (µm). A Microsoft Word file (Microhardness test.docx) contains the results of the microhardness test. The results are expressed as Young’s modulus values (GPa) presented in tables for each sample. A Microsoft Excel file (MIP - Porosity.csv) contains the results of porosity evaluation for each tested granule.



Uniwersytet Medyczny w Lublinie


Biomaterials, Regenerative Medicine, Polymer Nanocomposites, Bone Regeneration


Narodowe Centrum Nauki