Data Figure2_EC50_Monoclonal antibodies binding data for SARS-CoV-2 proteins
Data for figure 2. Data were processed. Data points and the fitting points were included in the raw data in excel format. The data were fit to standard four-parameter logistic equations using GraphPad Prism (GraphPad Software, La Jolla, CA).
Steps to reproduce
384-well microplates were coated overnight at 4 oC with 2 μg/mL protein or GST/MHT control proteins in PBS pH 7.4. Wells were blocked with 0.2% BSA in PBS for one hour and washed 4 times with 0.05% Tween in PBS (PT buffer). Serial diluted IgGs were into PBT transferred to antigen-coated plates and incubated at room temperature for 30 min, washed with PBST four times, and incubated for 30 min with anti-β-HRP antibody conjugate (1:7500 dilution in PBT). The plate was washed, developed, and read as described above. The data were fit to standard four-parameter logistic equations using GraphPad Prism (GraphPad Software, La Jolla, CA) and EC50 values were estimated.