Data Figure2_EC50_Monoclonal antibodies binding data for SARS-CoV-2 proteins

Published: 27 April 2022| Version 2 | DOI: 10.17632/scs64fxr4d.2
Contributors:
Nawneet Mishra,
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Description

Data for figure 2. Data were processed. Data points and the fitting points were included in the raw data in excel format. The data were fit to standard four-parameter logistic equations using GraphPad Prism (GraphPad Software, La Jolla, CA).

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Steps to reproduce

384-well microplates were coated overnight at 4 oC with 2 μg/mL protein or GST/MHT control proteins in PBS pH 7.4. Wells were blocked with 0.2% BSA in PBS for one hour and washed 4 times with 0.05% Tween in PBS (PT buffer). Serial diluted IgGs were into PBT transferred to antigen-coated plates and incubated at room temperature for 30 min, washed with PBST four times, and incubated for 30 min with anti-β-HRP antibody conjugate (1:7500 dilution in PBT). The plate was washed, developed, and read as described above. The data were fit to standard four-parameter logistic equations using GraphPad Prism (GraphPad Software, La Jolla, CA) and EC50 values were estimated.

Institutions

Uppsala Universitet, Washington University in Saint Louis School of Medicine, Boston University School of Medicine, University of Toronto

Categories

Biochemical Assay

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