Source data for unprocessed immuno-blots in manuscript CELL-STEM-CELL-D-19-00624

Published: 6 January 2021| Version 1 | DOI: 10.17632/sfpv2m95s6.1
Xiaoling Li


In this study, we investigated the role of histone crotonylation in regulation of linage specification of human embryonic stem cells. We showed that histone crotonylation is crucial for meso/endoderm differentiation by enhancing mesodermal and endodermal gene expression. Consistently, chemical enhancement of histone crotonylation promotes endoderm differentiation of hESCs, whereas deletion of crotonyl-CoA producing enzymes reduces histone crotonylation and impairs meso/endoderm differentiation in vitro and in vivo.