Sahu, Villa et al.

Description

The data in this study were derived from a variety of sources, including various cell lines, mice, and a yeast strain (Saccharomyces cerevisiae), all of which are comprehensively detailed in the main text and the supplemental materials and methods document. Our assessment of protein levels and metabolic activity was conducted through biochemical methods such as immunoblotting and mass spectrometry (metabolomics). To quantify the levels of proteins of interest, we followed the purification procedures outlined in the manuscript (Sahu, Villa, et al.), subsequently running them on SDS-PAGE gels. These gels were then transferred onto nitrocellulose membranes and subjected to hybridization with specific antibodies for chemiluminescent detection. In the case of metabolite extraction, cultured cells or segments of mouse livers were either scraped or ground into the indicated solutions specified in the supplemental methods. The resulting metabolite suspensions were then prepared for mass spectrometry analysis. Furthermore, we captured images of cells and lipid droplets by employing an optical microscope after staining them with Oil Red O. The files are systematically organized to align with the sequence of data presentation in the manuscript. Each image file is available in PDF format and contains the original, untreated images of western blots, cells, and figures, encompassing both those featured in the main text and supplementary materials. Additionally, the numerical data points for each graph in both the main and supplementary figures have been meticulously cataloged in separate Excel files, corresponding to the respective figure panels for ease of reference.

Institutions

Categories

Funding

Licence