Raw Data on Different Salinity Effects on the Cultivation of Procholoron sp.
Description
The data in this article describes the effect of different salinities on the growth of Prochloron cells outside hosts. The cultivation was performed in enriched standard culture media with continuous photoperiod. The culture stock of Prochloron cells which was made as inocula (starter) in the laboratory was isolated from tunics of an ascidian Lissoclinum patella.
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Steps to reproduce
The ascidian was obtained from 20 m depth at Malalayang coastal water in Manado Bay and kept on liquid media with salinity of 20 ppt. Then the cells were transferred into culture chambers, each consisted of different salinities:15, 20, 25, 30, 35, 40 and 45 ppt. After the cells reached exponential phase, some drops of cell suspension were transferred into agar media with the same salinity level until green colony appeared. Each of the colonies was transferred again into liquid media with the same salinity. Population growth was observed until the dead phase.