Data for: Regulation and Function of Gill CRF System during Osmotic Disturbances in Atlantic salmon

Published: 14 June 2024| Version 1 | DOI: 10.17632/shs4kg7vc7.1
Contributor:
Brett Culbert

Description

Supporting data for "Regulation and function of the gill corticotropin-releasing factor system during osmoregulatory disturbances in Atlantic salmon". Tab 1: CRF System Component Distribution in gills (qPCR) Tab 2: Effects of smolting on CRF System Component abundance in the gills Tab 3: Effects of FW-to-SW transfer on CRF System Component abundance in the gills Tab 4: Effects of SW-to-FW transfer on CRF System Component abundance in the gills Tab 5: Effects of cortisol on CRF System Component abundance in cultured gill filaments Tab 6: Effects of CRFa or UCN3 on transcript abundance in cultured gill filaments from FW-adapted fish as determined by RNA-Seq Tab 7: Effects of CRFa or UCN3 on transcript abundance in cultured gill filaments from SW-adapted fish as determined by RNA-Seq Tab 8: qPCR confirmation of effects of CRFa and UCN3 on transcript abundance in cultured gill filaments from FW-adapted fish Tab 9: Effects of FW- vs SW-acclimation on CRF receptor abundance in cultured gill filaments as determined via qPCR Tab 10: Effects of CRFa w or wo a CRFR1 antagonist on transcript levels of target genes in cultured gill filaments as determined via qPCR Tab 11: Effects of CRFa dose on PKA substrate phosphorylation as determined via western blotting (Supplementary data) Tab 12: Effects of CRFa doses on target gene expression in landlocked source population as determined via qPCR (Supplementary data)

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Institutions

University of Guelph

Categories

Endocrinology, Osmoregulation, Teleost, Transcriptomics, Corticotropin-Releasing Factor

Funding

Natural Sciences and Engineering Research Council of Canada

RGPIN-2015-04498

Genome Canada

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