Cenobamate exhibits prophylactic and therapeutic efficacy against cisplatin-induced peripheral neuropathy in rats: Behavioral and mechanistic insights
Description
This dataset contains raw behavioral and immunohistochemical data supporting the study titled "Cenobamate exhibits prophylactic and therapeutic efficacy against cisplatin-induced peripheral neuropathy in rats: Behavioral and mechanistic insights." Behavioral data include measurements of pain sensitivity and motor function obtained from rat models of cisplatin-induced peripheral neuropathy (CIPN). Sensory assessments include mechanical allodynia (dynamic plantar aesthesiometer), cold allodynia (acetone drop test), and thermal hyperalgesia (Hargreaves test). Motor function assessments include rotarod performance and footprint gait analysis. Immunohistochemical data include quantitative H-score measurements for brain-derived neurotrophic factor (BDNF), cleaved caspase-3, and interleukin-1β (IL-1β) in dorsal root ganglia (DRG) and sciatic nerve (SN) tissues. The dataset includes Excel files containing raw measurements and processed values used for statistical analysis. Data correspond to both prophylactic and therapeutic experimental protocols evaluating cenobamate effects compared with cisplatin and gabapentin-treated groups.
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Data were generated from a preclinical rat model of cisplatin-induced peripheral neuropathy (CIPN) designed to evaluate the prophylactic and therapeutic effects of cenobamate. Peripheral neuropathy was induced in adult male Sprague–Dawley rats by intraperitoneal administration of cisplatin (3 mg/kg/week) for five consecutive weeks. Two experimental protocols were conducted: a prophylactic protocol evaluating preventive effects during cisplatin exposure, and a therapeutic protocol evaluating treatment effects after neuropathy establishment. Behavioral data were collected using standardized sensory and motor function assays. Mechanical allodynia was assessed using a dynamic plantar aesthesiometer, recording paw withdrawal latency (PWL) and paw withdrawal threshold (PWT). Cold allodynia was evaluated using the acetone drop test, and thermal hyperalgesia was assessed using the Hargreaves thermal plantar test. Motor coordination and balance were assessed using the rotarod test, while gait analysis was performed using the footprint test to measure stride length. Following behavioral testing, animals were euthanized and dorsal root ganglia (DRG) and sciatic nerve (SN) tissues were collected for histological and immunohistochemical analyses. Immunohistochemistry was performed to evaluate brain-derived neurotrophic factor (BDNF), cleaved caspase-3, and interleukin-1β (IL-1β) expression. Staining intensity and percentage of positive cells were quantified using the H-score method. Raw measurements were recorded in Microsoft Excel spreadsheets and processed for statistical analysis using SPSS software. All instruments and procedures followed established experimental protocols commonly used in neuropathy research.