tweezercalib 2.0: Faster version of MatLab package for precise calibration of optical tweezers

Published: 15 March 2006| Version 1 | DOI: 10.17632/sjtgzs42rc.1
Poul Martin Hansen, Iva Marija Tolić-Nørrelykke, Henrik Flyvbjerg, Kirstine Berg-Sørensen


Abstract We present a vectorized version of the MatLab (MathWorks Inc.) package tweezercalib for calibration of optical tweezers with precision. The calibration is based on the power spectrum of the Brownian motion of a dielectric bead trapped in the tweezers. Precision is achieved by accounting for a number of factors that affect this power spectrum, as described in vs. 1 of the package [I.M. Tolić-Nørrelykke, K. Berg-Sørensen, H. Flyvbjerg, Matlab program for precision calibration of optical tweezer... Title of program: tweezercalib Catalogue Id: ADTV_v2_0 Nature of problem Calibrate optical tweezers with precision by fitting theory to experimental power spectrum of position of bead doing Brownian motion in incompressible fluid, possibly near microscope cover slip, while trapped in optical tweezers. Thereby determine spring constant of optical trap and conversion factor for arbitrary-units-to-nanometers for detection system. Versions of this program held in the CPC repository in Mendeley Data ADTV_v1_0; tweezercalib; 10.1016/j.cpc.2004.02.012 ADTV_v2_0; tweezercalib; 10.1016/j.cpc.2005.11.007 ADTV_v2_1; tweezercalib; 10.1016/j.cpc.2006.07.009 This program has been imported from the CPC Program Library held at Queen's University Belfast (1969-2018)



Optics, Molecular Biology, Statistical Physics, Biological Sciences, Computational Physics, Thermodynamics, Computational Method