Supplemental: Two photon microscopy allows for rapid processing of large Mohs specimens

Published: 16 December 2024| Version 2 | DOI: 10.17632/smbphsch6z.2
Contributor:
Connor Heckman

Description

Mendeley Supplemental Figure 1: A region of a two-photon image of a negative BCC Mohs surgery sample stained with AO/SYBR Green(nuclei) and SR101 (protein) (A) along with a zoomed region containing a hair follicle (B). The corresponding H&E stained FSA section for the specimen (C) along with a zoomed region (D) containing the same hair follicle shown above (B). Here we see great coregistration between the two zooms of the hair follicle between the MPM and H&E images. Mendeley Supplemental Figure 2: A region of a two-photon image of a negative BCC Mohs surgery sample stained with AO/SYBR Green(nuclei) and SR101 (protein) (A) along with a zoomed region containing sebaceous glands (B). The corresponding H&E stained FSA section for the specimen (C) along with a zoomed region (D) containing the same sebaceous glands shown above (B). Here we see great coregistration between the two zooms of the sebaceous gland between the MPM and H&E images.

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Institutions

University of Rochester

Categories

Biomedical Engineering, Microscopy, Fluorescence Microscopy, Mohs Surgery, Laser-Fluorescence Microscopy

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