BAS chromatin remodeler mediates controls brassinosteroid-induced transcriptional activation and plant growth in Arabidopsis (Western blot)
Description
Brassinosteroid (BR) signaling leads to the nuclear accumulation of the BRASSINAZOLE-RESISTANT 1 (BZR1) transcription factor, which plays dual roles in activating or repressing the expression of thousands of genes. BZR1 represses gene expression by recruiting histone deacetylases, but how it activates transcription of BR-induced genes remains unclear elusive. Here, we show that BR reshapes genome-wide chromatin accessibility landscape, increasing the accessibility of BR-induced genes, and reducing the accessibility of BR-repressed genes in Arabidopsis. BZR1 physically interacts with BRAHMA (BRM), the ATPase subunit of BRAHMA-Associated SWI/SNF (BAS) chromatin-remodeling complex, on the genome and selectively recruits the BAS complex to BR-activated genes. Depletion of BAS abrogates the capacities of BZR1 to increase chromatin accessibility, activate gene expression, and promote cell elongation, without affecting BZR1’s ability to reduce chromatin accessibility and expression of BR-repressed genes. Together, these data identify reveal that BZR1 recruits the BAS complex to open chromatin and to mediate BR-induced transcriptional activation of growth-promoting genes.