Data for Periodic crossbreeding between laboratory and wild populations enhances fitness in Helicoverpa zea (Boddie)
Description
This dataset provides detailed information on the life cycle and reproductive parameters of Helicoverpa zea, an economically significant agricultural pest. Data were collected under controlled laboratory conditions and are organized into three Excel files, which include: Life cycle duration by generation: Data on the duration of each developmental stage (egg, larva, pupa, adult) across three consecutive generations (T1-G1, T2-G2, T3-G3). Oviposition by treatment: Information on the number of eggs laid by individuals in each treatment and generation. Pupal weights: Individual pupal weights by treatment and generation, with basic statistical analyses. Purpose: This dataset is designed for studies on integrated pest management, genetic crossbreeding, and comparative statistical analysis. It provides a foundation for understanding how periodic crossbreeding improves the adaptability and biological performance of laboratory-reared populations. Format: The files are in Excel format and organized to facilitate statistical analysis using tools such as SPSS, R, or Python. License: This dataset is available under a Creative Commons Attribution (CC BY) license, allowing its use and adaptation with proper attribution.
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Steps to reproduce
Steps to Reproduce [1] Field Collection: Specimens of Helicoverpa zea were collected from maize fields in the Goyena community area (12°28'02.6"N 86°57'10.7"W). Third and fourth instar larvae were gathered due to their prevalence and significance in pest management. [2] Quarantine Period: The collected larvae underwent a quarantine process for two consecutive generations (G1 and G2) at the Biological Control Research and Reproduction Center (CIRCB). This step ensured acclimatization and elimination of potential contaminants. Experimental Setup: After quarantine, larvae were assigned to three treatments: T1-G1 (Generation I), T2-G2 (Generation II), and T3-G3 (Generation III). A completely randomized design was used, with 30 larvae per treatment, totaling 90 larvae per generation. Diet Preparation and Maintenance: Larvae were fed a soybean-based diet supplemented with vitamins and antibiotics to ensure optimal growth conditions. Each larva was placed in an individual cup containing a 2-cm square of sterilized diet. [3] Data Collection: Key variables were measured daily: Larval length (mm) using a millimeter grid. Larval and pupal weights using an analytical balance. Egg hatching rates via visual counts. Lifecycle duration for each developmental stage (egg, larva, pupa, adult). Statistical Analysis: Data were analyzed using IBM SPSS Statistics v.21. Statistical tests included ANOVA, Kolmogorov-Smirnov, Shapiro-Wilk, and Mann-Whitney U, as appropriate, to compare treatments and validate the results.