The acute effects of ultraviolet radiation on the blood transcriptome are independent of plasma 25OHD3

Published: 14 September 2017| Version 1 | DOI: 10.17632/snwxktgxpz.1
Contributors:
Mariona Bustamante,
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Description

Blood gene and miRNA expression before (0h) and after (6h, 24h and 48h) exposure to whole body ultraviolet radiation. Data in "ICE_blood_data_Mendeley.xlsx" file, contains: - Sheet "mRNAseq_samples": samples after QC in the mRNAseq analysis - Sheet "mRNAseq_expr_matrix": gene expression - mRNAseq non normalized counts - Sheet "small RNAseq_samples": samples after QC in the small RNAseq analysis - Sheet "small_RNAseq_matrix": miRNA expression - small RNAseq non normalized counts

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Design: -9 male volunteers skin type I/II, from UK -UVR expsoure: -- whole body fluorescent solar simulated radiation (FSSR) -- dose: 2.5 - 3 standard erythema dose (SED) -- 5 in March-April and 4 in July-September -blood collected at 0h (unexposed), 6h, 24h and 48h RNA: -Blood collected in PAXGene tubes -RNA extraction with PAXgene blood RNA kit (Qiagen, Hilden, Germany) Sequencing: -TruSeq RNA Sample Prep Kit v2 (mRNA) (Illumina, San Diego, California, USA) -TruSeq Small RNA Sample Prep Kit (small RNA) (Illumina, San Diego, California, USA) mRNAseq QC: -Mapping with R package Rsubread, allowing a maximum of 5 mismatches and using the hs37d5 as reference. -Gene annotation with NCBI hg19 (Entrez Gene) database. -Sample size after QC: 36 miRNAseq QC: -As previously described (Lappalainen et al., 2013). -Sample size after QC: 35 (one excluded because contaminated) Differential analysis: -R package DESeq2 v.1.10.1

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Gene Expression

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