TPM RNA-seq data of HCC xenograft tissues treated with or without nitidine chloride

Published: 10 December 2021| Version 1 | DOI: 10.17632/stkrjf7w7t.1


In recent times, traditional Chinese medicine has gained popularity in the clinical management of human cancers due to its anti-tumor pharmacological action, synergism with chemotherapy and toxity-reducing effect. Nitidine chloride (NC) is a natural alkaloid extracted from the root of traditional Chinese herb Zanthoxylum nitidum and we have previously discovered the tumor-combating activities of NC in HCC. It would be interesting to analyze the influence of NC on expression profiles of genes in HCC. Herein, in vivo experiment was carried out on nude mice for evaluating changes in gene expression profiles from xenograft of HCC tissues before and after the treatment of NC. The animal experiments were performed according to the Guide for the Care and Use of Laboratory Animals (the Shanghai SLAC Laboratory Animal of China, 2015) and were approved by the Ethics Committee of the First Affiliated Hospital of Guangxi Medical University (Nanning, China). BALB/c nude mice were purchased from Guangxi Medical University Animal Center (Guangxi, China). The mice were housed in pathogen-free conditions with a 12 h light/12 h dark cycle at room temperature. SMMC7721 cells (1 × 107 cells/L) were injected into the right armpit of each mouse. Fourteen days later, mice with a tumour volume of approximately 70 mm3 were randomized into two groups: The negative control group containing three mice was intraperitoneally injected with saline, and the NC group containing three mice was intraperitoneally injected with 7 mg/kg/d NC. After 15 days of drug administration every other day, the mice were euthanized and the tumour tissues were excised and stored at -80℃. MRNA-sequencing was conducted following the procedures of NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB, USA).



Guangxi Medical University


RNA Sequencing