Investigating the mechanism of action of aggregation-inducing antimicrobial Pept-ins

Published: 03-12-2020| Version 1 | DOI: 10.17632/sxy5xknpm5.1
Contributor:
Guiqin Wu

Description

Raw microarray data, the data set is related to figure 4.

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Four independent strains of ancestors and P2-resistant cells were selected from the overnight culture prepared for lipidomic analysis. Overnight culture was diluted with fresh MH medium to 0.5 McF, 20 mL of which was either treated with vehicle or P2 (3.25 μg/mL) for 4 h at 37 °C with shaking. Treated bacterial culture was collected by centrifugation (7 000 rpm, 4 min) and the pellet was stored at -80 °C before shipping. RNA isolation and transcriptomic analysis were carried out by Oaklabs (Berlin, Germany). Briefly, fluorescent complementary RNA (cRNA) was generated from high-quality total RNA by using low Input QuickAmp Labeling Kit (Agilent Technologies). cDNA synthesis was performed by using WT primer. Labelled cDNA was hybridised on 8 x 60K glass slide using agilent gene expression hybridisation kit (Agilent Technologies). Fluorescence signals on microarrays are detected by the SureScan Microarray Scanner (Agilent Technologies), generating a 20 bit TIFF file. This file was read and processed using Agilent’s Feature Extraction software version 11.