CryoSEM images showing secondary cell wall macrofibrils in seed plants

Published: 4 October 2023| Version 2 | DOI: 10.17632/sy5whnf72f.2
Raymond Wightman,


Fresh woody material was harvested from twenty six plant species across gymnosperms and angiosperms and frozen in slush N2, cryo-fractured, sputter coated with a measured 3 nm platinum layer and then imaged at high magnification on a cryoSEM (Zeiss EVO equipped with Quorum PP3010 cryo system). Each image shows the wood ultrastructure of vessels/tracheids including individual cellulose macrofibrils. These data are used to measure the thickness of the macrofibrils from each species. Further details of the specimens that were sampled are shown in the associated Table 1 "Accession details".


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CryoSEM was carried out as published in "Structural Imaging of Native Cryo-Preserved Secondary Cell Walls Reveals the Presence of Macrofibrils and Their Formation Requires Normal Cellulose, Lignin and Xylan Biosynthesis"


Uniwersytet Jagiellonski w Krakowie Wydzial Biochemii Biofizyki i Biotechnologii, University of Cambridge Sainsbury Laboratory


Evolutionary Biology, Plant Biology, Wood, Conifer, Angiosperm, Plant Cell Wall


Narodowe Centrum Nauki

Gatsby Charitable Foundation