The effect of genetic and environmental perturbations on S. cerevisiae cell size

Published: 9 November 2018| Version 1 | DOI: 10.17632/t4n9mys2sm.1
Contributors:
Felix Jonas,
Ilya Soifer

Description

each cell cycle file contains all compromises all cell cycles in a single structure with several fields: mother_ck_size: mother cell size at the cytokinesis of the previous cell cycle. mother_ns_size: mother cell size at the nuclear separation of the previous cell cycle. mother_bud_size: mother cell size at budding in the current cell cycle. mother__final_ck_size: mother cell size at the cytokinesis of the current cell cycle. mother_final_ns_size: mother cell size at the nuclear separation of the current cell cycle. daughter__final_ck_size: daughter cell size at the cytokinesis of the current cell cycle. daughter_final_ns_size: daughter cell size at the nuclear separation of the current cell cycle. ns_time: timepoint of the nuclear separation at the previous cell cycle ck_time: timepoint of the cytokinesis at the previous cell cycle bud_time: timepoint of budding (G1 to S transition) in the current cell cycke final_ns_time: timepoint of the nuclear separation at the current cell cycle final_ck_time: timepoint of the cytokinesis at the current cell cycle n_cycles: number of the current cycles for the mother (i.e. 1 = first cell cycle after birth). cell size is in pixels

Files

Steps to reproduce

In order to reproduce the results of Jonas et al. 2018, it is necessary to analyse only the first cell cycle (n_cycles=1) for eachdataset. Moreover, excluding cells born less than 120 minutes before the end of the movie (360 minutes), reduces the biases towards fast-dividing cells.