Sadovoe
Description
Data on physio-chemical properties of soils and its elemental composition
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Soil samples (500 – 700 g each) were collected from a depth 0 – 130 cm (A, B and C soil horizons of Kastanozems and Solonetz). Plastic and steel tools were used for sampling. After air-drying and declumping the aggregates, the soil was sieved through a 1 mm mesh sieve. In soil samples, we measured particle-size distribution, elemental composition (total concentration of 26 chemical elements), and total organic carbon content (dichromate digestion based on Walkley-Black method). The particle-size distribution was analyzed using a laser diffraction technique and an ‘Analysette 22’ equipment (Germany) in samples pre-treated with 4% Na4P2O7. CaCO3 concentration was analyzed by a manometric measurement of the CO2 released following acid (HCl) dissolution.The total content of chemical elements was measured using an X-Ray fluorescence technique and a PANalytical spectrometer (Netherlands). We prepared a soil water extracts to measure pH value (1:2.5 soil:water ratio) and electrical conductivity (a 1:5 soil:water ratio). Mobile fractions (F1 – F3) were obtained according to the extraction procedure by [11] with the use of the following reagents: F1 (ChE1) – with NH4Ac (ammonium acetate buffer) and the soil:solution ratio of 1:5, F2 (ChE2) – with 1% EDTA (ethylenediaminetetraacetic acid) in NH4Ac and the soil:solution ratio of 1:5 and F3 (ChE3) – with 1M HNO3 and the soil:solution ratio of a 1:10. Concentrations of the extracted ChEs in the filtrates were determined using an ‘iCAP-6500’ (Inductively Coupled Plasma Atomic Emission Spectrometer by ‘Thermo Scientific’, USA). The ChE mobility (ChE_m, Table S1) was calculated as a ratio of its mobile fractions (F1+F2+F3) to its total content, multiplied by 100%.