Metagenomic dataset of flesh-footed shearwater (Ardenna carneipes)

Published: 6 April 2021| Version 2 | DOI: 10.17632/t78p5tgvrc.2
Subir Sarker,
, Jennifer Lavers


Source of sampling and extraction of DNA A cutaneous tissue sample was collected from Flesh-footed Shearwater originating from Lord Howe Island, New South Wales, located approximately 500 km off the east coast of Australia during April 2015 (sample ID15-1527, GPS location: 32.53° S, 159.08° E). The sample was collected with the permission of the Lord Howe Island Board (permit no. LHIB 02/14) under the approval of the University of Tasmania and Charles Sturt University Animal Ethics Committees (permit no. A0010874, A0011586, and 09/046). The genomic DNA was isolated according to our previously established protocol (Sarker et al., 2017) using the Qiagen Blood and Tissue mini kit (Qiagen, Germany). The extracted DNA has been stored at -20 °C for further testing at Charles Sturt University. Sequencing, assembly and annotation of complete mitogenome of A. carneipes The library preparation and sequencing was performed as previously described (Sarker et al., 2017). Briefly, the paired-end library with an insert size of 150 bp was prepared using the Illumina paired-end sample preparation kit (Illumina, San Diego, CA) according to the manufacturer's instructions. Sequencing of the pooled DNA-library was performed on a HiSeq4000 sequencing platform (Illumina) according to the manufacturer’s instructions by Novogene, China. Sequencing data used in this study were analysed according to the previously established pipeline (Sarker et al., 2017; Sarker et al., 2019a; Sarker et al., 2019b) using Geneious (version 10.2.2, Biomatters, New Zealand) and CLC Genomics Workbench (version 9.5.4). Briefly, a total of 14.42 million reads with a read length of 150 bp was used to obtain the complete mitochondrial genome of A. carneipes.



Metagenomics, Seabird