Ginseng polysaccharides promote intestinal epithelial cell migration through polyamine and HuR regulatory mechanisms
Description
Ginseng, an important Chinese medicine, is thought to benefit the qi and strengthen the spleen. Herein, we sought to investigate the mechanism behind ginseng polysaccharide-induced stimulation of IEC-6 cell migration, and the role of polyamines. Combined with intervention data for ginseng polysaccharide on dextran sodium sulfate- (DSS) induced colonic lesions in mice, we also studied the mechanism behind ginseng-mediated repair of intestinal mucosal damage. To this end, mice were fed 3%(w/v) DSS for 6 d to create a colonic lesion model and a cell migration model was created using the cell scratching method. Subsequently, mRNA expression, protein expression, mRNA translation efficiency, and nucleoplasmic distribution of HuR were determined by qRT-PCR, western blot, a dual luciferase reporter gene system, and by immunofluorescence, respectively. We found that ginseng polysaccharide-induced repair of intestinal mucosal injury may be mediated by increased cell migration via polyamine regulatory mechanisms. In vitro and in vivo experiments suggest that ginseng polysaccharide-induced post-transcriptional regulation of STIM1, Cdc42, and Rac1 is likely also implicated, and may be related to differences in the regulation of different target genes by HuR. Taken together, these data provide a reference for further exploration of the protective effect of ginseng on the intestinal mucosa