RNA-seq on C2C12 myoblasts treated with 1 uM of 20-hydroxyecdysone for 24 hours. Part 2. 20E-treated samples.

Description

This dataset is a part 2 of a project on RNA-seq data of C2C12 myoblasts treated with 1 uM of 20-hydroxyecdysone (ecdysterone) for 24 hours. This dataset contains only 20E-treated samples (7-12CIN) Device - NextSeq2000 Illumina Reads - 51 cycles Kit - TruSeq Stranded mRNA Illumina Indexes - IDT for Illumina TruSeq RNA UD Indexes For RNA-seq analysis, C2C12 myoblasts were treated with 1 μM of 20E for 24 hours. The control cells were treated with DMSO. Six biological replicates for each sample were used. Total RNA was extracted using the MagNA Pure Compact RNA Isolation Kit (Roche, Switzerland) on a MagNA Pure Compact instrument (Roche). RNA concentration was measured using a Qubit 4 fluorometer (Thermo Fisher Scientific, USA); RNA quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). The RNA integrity number (RIN) of the isolated RNA was ≥ 9.5. cDNA libraries were prepared from 1 μg of total RNA using the TruSeq Stranded mRNA Library Prep Kit (Illumina, USA) according to the manufacturer's instructions. Libraries were sequenced on an Illumina NextSeq 2000 System in single-read mode (51 bp). A minimum of 20 million reads were generated for each sample. Sample information: 7CIN - 20E-treated (1 uM, 24h), replicate 1; 8CIN - 20E-treated (1 uM, 24h), replicate 2; 9CIN - 20E-treated (1 uM, 24h), replicate 3; 10CIN - 20E-treated (1 uM, 24h), replicate 4; 11CIN - 20E-treated (1 uM, 24h), replicate 5; 12CIN - 20E-treated (1 uM, 24h), replicate 6.

Steps to reproduce

For RNA-seq analysis, C2C12 myoblasts were treated with 1 μM of 20E for 24 hours. The control cells were treated with DMSO. Six biological replicates for each sample were used. Total RNA was extracted using the MagNA Pure Compact RNA Isolation Kit (Roche, Switzerland) on a MagNA Pure Compact instrument (Roche). RNA concentration was measured using a Qubit 4 fluorometer (Thermo Fisher Scientific, USA); RNA quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). The RNA integrity number (RIN) of the isolated RNA was ≥ 9.5. cDNA libraries were prepared from 1 μg of total RNA using the TruSeq Stranded mRNA Library Prep Kit (Illumina, USA) according to the manufacturer's instructions. Libraries were sequenced on an Illumina NextSeq 2000 System in single-read mode (51 bp). A minimum of 20 million reads were generated for each sample.

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