Database for article describing the molecular profile of perinatal induction with MSG and the consequences for skeletal muscle

Published: 27 June 2022| Version 1 | DOI: 10.17632/td245zm5wg.1
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Description

The data used for the elaboration of the scientific article referring to the description of the molecular and biochemical changes of the perinatal induction of MSG for a characteristic model of obesity and its influences on body characteristics, plasma profile, skeletal muscle structure, fiber types profile , neuromuscular junction profile and extracellular matrix composition. The animals used showed inflammatory and molecular changes that prove that the change in muscle structure is due to perinatal disturbance, that is, phenotypic programming, and not only due to the chronicity of the obesogenic condition and metabolic syndrome as classically described.

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From postnatal day (PN) 1 to PN5, male Wistar rats (n = 24) received daily subcutaneous injections of MSG solution in the dorsocervical region (4 mg. g -1 body weight, MSG group) or equimolar saline solution (1 .25 mg g − 1 body weight, control group - CTL) (Olney 1969; de Andrade et al. 2021). Every two days, the animals were weighed and the nasoanal length was measured, until the 14th day of life. In PN15, 12 animals were eu-thanized (n = 6 per group) to assess the establishment of molecular damage. After wean-ing (PN21), food consumption and the evolution of body weight were monitored weekly. All animals were housed in standard cages at constant temperature (22 ± 1 °C), on a 12 h light-dark cycle, and had ad libitum access to water and standard laboratory chow (Bi-oBase®, Santa Catarina, Brazil). In PN15, the Lee index (∛bodyweight / nasal-anal length x 1000) was calculated. The animals were then desensitized in a carbon dioxide chamber and then euthanized by de-capitation [7]. Blood was collected in heparinized tubes and centrifuged at 4 °C, at 12,000 RCF (g) for 10 min to measure the plasma biochemical and inflammatory profile. the ab-dominal wall and pelvic limb muscles were collected (approximately 0.2 g) and intended for the analysis of oxidative damage markers and Western blotting In PN142, the Lee index (∛bodyweight / nasal-anal length x 1000) was calculated. The animals were then desensitized in a carbon dioxide chamber and then euthanized by decapitation [7]. Retroperitoneal, Perigonadal, and brown fats were removed, weighed, normalized to g.100 g − 1 of body weight, and used to calculate body adiposity [2]. Blood was collected in heparinized tubes and centrifuged at 4 °C, at 12,000 RCF (g) for 10 min to measure the plasma biochemical profile. The extensor digitorum longus (EDL) and soleus muscle (SOL) was dissected, collected, weighed, measured, and destined for biochemical and morphological analysis.

Institutions

Universidade Federal do Parana

Categories

Mammalian Target of Rapamycin, Neuromuscular Junction, Skeletal Muscle Structure, Metabolic Syndrome, Biological Development

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