Data on the effects of environmentally relevant concentrations of glyphosate and aminomethylphosphonic acid on biotransformation and redox proteins in the liver of zebrafish (Danio rerio)

Published: 21 November 2024| Version 1 | DOI: 10.17632/td75wv39fk.1
Contributors:
Alexey Morozov, Victoria Yurchenko

Description

The dataset presents the log2(FC) and p values for the biotransformation and redox proteins in the liver of zebrafish D. rerio.

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Steps to reproduce

The proteome analysis was conducted at the “Human Proteome” Core Facility, Department of Proteomic Research, IBMC, Moscow, Russia, employing the label-free HPLC-MS/MS method (described in Morozov, Yurchenko (2024) [https://doi.org/10.1016/j.pestbp.2024.106036]). Label-free quantitative data sets were processed using Microsoft Excel, according to Aguilan et al. (2020) [https://doi.org/10.1039/D0MO00087F]. Briefly, LFQ intensities were log2-transformed, normalized by average and slope, and the missing values were replaced with the Probabilistic Minimum Imputation method. Processed proteomic data were used to calculate the relative abundance of each protein, i.e., the fold change (FC) between each treatment and control. Then, an F-test was performed between treatment and control; after that, based on the obtained p-value, a t-test type 3 or type 2 was performed. Only proteins with p < 0.05 are given in the dataset.

Categories

Proteomics, Redox Metabolism, Fish, Herbicide, Biotransformation, Glyphosate, Xenobiotics

Funding

Russian Science Foundation

23-26-00291

Licence