Photo-crosslinking proteomic analysis of IFITM3 interactors
Published: 21 February 2020| Version 1 | DOI: 10.17632/tds8by96gc.1
A diazirine-containing unnatural amino acid was site-specifically incorporated into IFITM3, an antiviral protein. After photo-irradiation, the interacting proteins were crosslinked with IFITM3, isolated by immunoprecipitation, and identified by quantitative SILAC proteomics.
Peking University Shenzhen Graduate School School of Chemical Biology and Biotechnology