Photo-crosslinking proteomic analysis of IFITM3 interactors

Published: 21 February 2020| Version 1 | DOI: 10.17632/tds8by96gc.1
Contributor:
Tao Peng

Description

A diazirine-containing unnatural amino acid was site-specifically incorporated into IFITM3, an antiviral protein. After photo-irradiation, the interacting proteins were crosslinked with IFITM3, isolated by immunoprecipitation, and identified by quantitative SILAC proteomics.

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