Brain temperature as an indicator of neuroinflammation induced by typhoid vaccine: assessment using whole-brain magnetic resonance spectroscopy in a randomised crossover study
Description
Typhoid vaccine was administered to 20 healthy participants in a randomised, placebo-controlled, crossover trial. Typhoid vaccine was used to induce a low level of neuroinflammation in participants, peaking at 3-4 hrs post-treatment. Magnetic resonance spectroscopic imaging (MRSI) in conjunction with echo-planar spectroscopic imaging (EPSI) was conducted at baseline and at 3-4 hrs post-treatment. Body temperature and mood, assessed using the Profile of Mood States (POMS), were measured at baseline and hourly thereafter up to 4 hrs post-treatment. The brain temperature and metabolite ratio data presented were processed using MIDAS software.
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The data includes baseline and post-treatment measures. Post-treatment measures refer to post-placebo (=0) and post-vaccine (=1). The study was performed in a crossover design, so each participant completed four MRI scanning sessions (two at baseline, one post-placebo, and one-post-vaccine). The complete methods for the trial will be published shortly and the link included here. The data includes metabolite ratios relative to creatine (CR), including myo-inositol (MI), lactate (LAC), choline (CHO), and n-acetylaspartate (NAA). Baseline and post-treatment TMAP and regional brain temperature measurements are also included. TMAP is an output from the open-source MIDAS software processing; the regional temperature is simply the TMAP value plus 32. Further information on TMAP is available in the MIDAS software documentation online. The metabolite ratios are also produced by the MIDAS software. Mood, assessed using the Profile of Mood States (POMS), and body temperature were measured every hour from baseline until 4 hrs post-treatment. Body temperature was measured in the right ear. Mood scores were calculated according to the POMS manual, resulting in 7 subscales of tension, depression, anger, fatigue, confusion, and vigour. Total mood disturbance was calculated by summing the negative mood subscales and then subtracting the positive mood subscale.