Credit41_In_Lab_Evolution

Published: 29 April 2020| Version 2 | DOI: 10.17632/ts5syfw38r.2
Contributor:
apoorva ravi shankar

Description

Different strains of Saccharomyces cerevisiae were treated with Credit41, then isolated and sequenced. Whole genome sequencing of GSY147(S288c backgroud), YJM789, AWRI1631 and RM11, grown in YPD, YM or YM supplemented with WYF, treated or not with Cr41, using Illumina Miseq. The cells were grown for 6 passages, then screened for resistance. A single colony of resistant cells was used for genomic DNA extraction using phenol-chloroform method (Hirt, 1967). The library was built using Quanta-bio sparq DNA frag and library kit and sequenced on an Illumina Miseq platform. Basecalls performed with Illumina’s FASTQ Generation (v1.0.0) available in BaseSpace. The data for all strains was then aligned to S288c reference (release R64-2-1) by creating an index using GATK-4.1.1.0. The GATK HaplotypeCaller was used to generate the vcf files.

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Read file uploaded as apravishankar_Credit41_ILE_submission.xlsx