Experimental Cavitation Data from ex vivo Normothermically Perfused Porcine Liver using Protein Cavitation Nuclei and Focused Ultrasound

Description

Calibrated array element signals (in units of Pa) received using an L7-4 linear array (ATL Ultrasound, Bothell, WA, USA) during a focused ultrasound treatment of a porcine liver using protein cavitation nuclei (pCaN) conducted on the 3rd May 2022. The MATLAB data file (Liver_PCaN_L74_calibrated_data_03May2022.mat) contains the following variables: Fs: data sampling frequency (25 MHz) Tp: Time stamp of each pulse [s] rfData: array element signals (number of array elements, number of samples, number of pulses) [Pa] what: description of the data.

Steps to reproduce

Cavitation was initiated using a single-element focused bowl ultrasound transducer (H107, Sonic Concepts, Bothell, WA, USA) within a healthy section of ex vivo porcine liver, using a commercially available system (OrganOx metra®, Oxford, UK) to achieve normothermic (37◦C) organ perfusion. 50 mL of ultrasound-responsive protein cavitation nuclei (pCaN) formulated using porcine serum albumin (PSA62, Equitech-Bio inc) using the method detailed by Hettinga et al [1] were injected intothe hepatic portal vein, followed by a 50 mL saline flush. The FUS transducer also had a 47 × 20 mm rectangular opening to facilitate mounting of a co-axially aligned L7-4 array transducer (ATL Ultrasound, Bothell, WA, USA), located 7.6 cm from the focus of the FUS transducer. This array was used to passively record cavitation signals using a configurable ultrasound array controller (Vantage 256, Verasonics). A calibrated scattering experiment was used to estimate and compensate for the combined element sensitivity and diffraction response following work by Gray et al [19]. Acoustic coupling between the transducers and the liver surface was achieved using a water cone and ultrasound gel. Ultrasound Parameters: 1.6 MPa peak negative pressure Fundamental frequency: 500 kHz Number of Cycles per pulse: 4000 Pulse Repetition Period: 2 s Experiment Timeline: 0-10s: background HIFU off, 0:16s: HIFU on 0:46s: 50 ml PCaN bolus start followed by 50 ml Saline flush References [1] J. K. Hettinga, B. Lyons, J. Balkaran, P. Rijal, D. Dunn-Lawless, L. Caproni, M. Gray, K. S. Suslick, C. C. Coussios, R. C. Carlisle, Cavitation-Mediated Transcutaneous Delivery of Protein and Nucleotide-based Antigen for Rapid High-level Immune Responses, Advanced Therapeutics 6 (12) (12 2023). doi:10.1002/adtp.202300102. [2] M. D. Gray, C. C. Coussios, Broadband Ultrasonic Attenuation Estimation and Compensation with Passive Acoustic Mapping, IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control 65 (11) (2018) 1997–2011. doi:10.1109/TUFFC.2018.2866171.

Institutions

• University of Oxford

Categories

Funders

• Donald Porteous

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