Basal cytokine secretion in human breast cancer cells and non-carcinogenic breast epithelial cells – 41-cytokine MILLIPLEX assay data

Published: 24 Oct 2019 | Version 2 | DOI: 10.17632/tvt8zm37w5.2
Contributor(s):
  • Karin Chen,
    Karin Chen
    Friedman Diabetes Institute at Lenox Hill Hospital, Northwell Health
  • Leo Satlof,
    Leo Satlof
    The Feinstein Institutes for Medical Research, Northwell Health
  • Udithi Kothapalli,
    Udithi Kothapalli
    The Feinstein Institutes for Medical Research, Northwell Health
  • Noah Ziluck,
    Noah Ziluck
    The Feinstein Institutes for Medical Research, Northwell Health
  • Maribel Lema,
    Maribel Lema
    Donald and Barbara Zucker School of Medicine at Hofstra/Northwell
  • Leonid Poretsky,
    Pharmacology, Toxicology and Pharmaceutical Science
    Friedman Diabetes Institute at Lenox Hill Hospital, Northwell Health
  • Dimiter Avtanski
    Medicine and Dentistry
    Friedman Diabetes Institute at Lenox Hill Hospital, Northwell Health

Description of this data

Human breast cancer cells (MDA-MB-231, MCF-7, T-47D, and BT-474) and non-carcinogenic breast epithelial (MCF-10A) cells were grown for 24 and 48 hours in 1 ml of serum-free medium. Using MILIPLEX assay kit (MilliporeSigma, Cat. # HCYTOMAG-60K), we measured the levels of the following cytokines in cell culture conditioned medium: EGF, FGF-2, TGFα, PDGF-AA, PDGF-AB/BB, TNFα, TNFβ, IFN-α2, IFN-γ, CCL2, CCL3, CCL4, CCL5, CCL7, CCL11, CCL22, CXCL1, CXCL10, CX3CL1, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17A, IL-1RA, G-CSF, GM-CSF, FLT3L, sCD40L, VEGF-A.

Experiment data files

Latest version

  • Version 2

    2019-10-24

    Published: 2019-10-24

    DOI: 10.17632/tvt8zm37w5.2

    Cite this dataset

    Chen, Karin; Satlof, Leo; Kothapalli, Udithi; Ziluck, Noah; Lema, Maribel; Poretsky, Leonid; Avtanski, Dimiter (2019), “Basal cytokine secretion in human breast cancer cells and non-carcinogenic breast epithelial cells – 41-cytokine MILLIPLEX assay data”, Mendeley Data, v2 http://dx.doi.org/10.17632/tvt8zm37w5.2

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Categories

Endocrinology, Oncology, Cytokines, Breast Cancer

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