polycystic ovary syndrome
Aim : explore pathophysiology of polycystic ovary syndrome. We have conducted a case control study. Polycystic ovary syndrome were diagnosis according to Rotterdam criteria. For all participants blood analysis were made on the third day of meases.
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We have conducted a case-control study . The southern and central regions of Tunisia are served by our hospital of Sfax-Tunisia. PCOS diagnosis was made based on Rotterdam criteria. Age and Body Mass Index (BMI) paired controls were recruited. Weight and height were measured and BMI was calculated. Exclusion criteria were: pregnancy, lactation, adrenal hyperplasia, androgen-secreting tumors, hyperprolactinemia, thyroid dysfunction, diabetes, all other known chronic disorders, and chronic drug intake. Fasting blood samples were collected on the third day of menses. Collection tubes have been centrifuged and run rapidly or stored at −80 ◦C until analyzed. 17-hydroxyprogesterone, androstenedione (A4), and dehydroepiandrosterone-sulfate (SDHEA) were analyzed by ELISA kit (DRG International, USA). All other hormonal assays: 25-hydroxyvitamin D (25vitD), follicle-stimulating hormone, luteinizing hormone, testosterone, prolactin, estradiol, and insulin were made by automated methods on Elecsys 2010 (Roche Diagnostics, Germany), and biochemical parameters (glucose and lipid profile) on Synchron CX9 (Beckman Coulter, USA).