Bioactive compound isolation from Uromyrtus metrosideros and their structure characterization

Published: 19 August 2022| Version 2 | DOI: 10.17632/ty35tfdhxc.2
Edita Ritmejerytė, Brendan Byatt, Norelle Daly, Stephen Pyne, Phurpa Wangchuk


Crude polar extract from Uromyrtus metrosideros leaves was fractionated using RP-HPLC. Absorbance data (at 254 nm) of the crude extract were extracted as "Crude extract HPLC data.csv" and plotted using R script "hplc plots.R". The purity of isolated compounds 1-6 was confirmed using analytical RP-HPLC at 254 nm (Fraction purity HPLC data) and plotted using R script "hplc plots.R" . Elemental analysis, high resolution mass spectrometry and 1D and 2D NMR experiments, namely 1H, 13C APT, 1H-13C HSQC, 1H-1H COSY, 1H-1H TOCSY, 1H-1H HMBC, 1H-1H NOESY were collected and used for structure elucidation (provided in "Compound details.docx" file). Raw NMR experimental data are provided in separate folder for each compound.



Phytochemicals, Nuclear Magnetic Resonance Spectroscopy, Plant-Derived Secondary Metabolite, Secondary Metabolite, Structural Studies on Chemical Compounds, Phytochemical Isolation, Natural Compound, Two-Dimensional Nuclear Magnetic Resonance Spectroscopy