Data from: Taxonomic accuracy and complementarity between bulk and eDNA metabarcoding provides an alternative to morphology for biological assessment of freshwater macroinvertebrates

Published: 7 March 2024| Version 1 | DOI: 10.17632/tys336jdsd.1
, Owen S. Wangensteen, Simona Somma, Leif Väisänen, Pau Fortuño,


Using broad range universal primers for the DNA barcode marker cox1 (Leray-XT, Wangensteen et al. 2018), we compare community composition captured between morphological and molecular-based approaches from different sources —tissue-based (bulk benthic and bulk drift samples) and environmental DNA (eDNA, filtered water) metabarcoding— for samples collected along a gradient of anthropogenic disturbances. Original Fastq files are deposited in the NCBI SRA repository. The ngsfilter file needed for demultiplexing the fastq files is shared here. Files can be demultiplexed using this ngsfilter table in OBITools ngsfilter command.



Universitat de Barcelona


Aquatic Invertebrate, Freshwater Ecology, Metabarcoding