matK and rbcL barcode sequences in a collection of Apulian glassworts

Description

The two datasets lists matK and rbcL barcodes sequenced in a small collection of glasswort ecotypes sampled in coastal wastelands, saltmarshes, and rocky coasts of Apulia region (southern Italy) . Samples were identified by combining symilarity-based DNA barcoding, Allele-Specific diagnostic PCR and phylogeny-based clustering, and assigned to annual (Salicornia europaea) and perennial (Sarcocornia fruticosa, Arthrocaulon macrostachyum ) species. MSSa = Sarcocornia fruticosa from Margherita di Savoia saltworks (BAT); VL = Sarcocornia fruticosa from Varano lake; SC = Salicornia europaea from Spirito Contadino commercial farm (FG); LROb = Salicornia europaea cultivated at Laguna del Re Oasis (FG); SEEDau = Salicornia europaea seeds purchased by Aurora local nursery (FG); SEEDeu = Salicornia europaea certified seeds supplied from an Irish seed company; MSSb = Arthrocaulon macrostachyum from Margherita di Savoia saltworks (BAT); MA-BC = Arthrocaulon macrostachyum from Monti Arena-Bosco Caggione (TA); LROa = Arthrocaulon macrostachyum from Laguna del Re Oasis (FG).

Steps to reproduce

matK and rbcL barcode sequences were used for a similarity-based search in NCBI and BOLD databases to perform a putative species identification of Apulian glassworts. matK and rbcL sequences were also used to infer evolutionary distances within Apulian ecotypes by implementing the Maximum Likelihood method with Kimura 2-parameter model in MEGA 11 software.

Institutions

• University of Foggia

  Apulia, Foggia

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