Mouse WOI Stroke Data

Published: 5 August 2022| Version 1 | DOI: 10.17632/v3jwyg7rcx.1
Contributors:
Lindsey Brier,

Description

Baseline imaging of two mice for five minutes on 11/18/22 ('201118') right before photothrombosis to left somatosensory cortex (2.2mm left and 0.5mm anterior to bregma with a 0.5mm spot size). Same imaging performed on same two mice for five minutes three days later 11/22/20 ('201122'). Each MATLAB file is 156 x 156 x 20160 representing pixels x pixels x frames. Each frame consists of an image of the dorsal cortical surface of each Thy1-GCaMP6f expressing mouse. Framerate of acquisition was 16.8Hz per LED channel. Four sequential LED channels were used; blue (470nm) for GCaMP6 excitation, green (530nm), yellow (590nm), and red (625nm) for hemoglobin spectroscopy. Data processing and analytic tools described in Brier and Culver (BioRxiv, April 2021).

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Institutions

Washington University in Saint Louis

Categories

Optical Imaging, Mouse Study, Calcium Imaging

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