20250124_TUM_BioSE_DR

Published: 24 January 2025| Version 1 | DOI: 10.17632/vcccx3k7mv.1
Contributors:
Dennis Roecker,
,
,
,
,

Description

*A novel approach utilizing electrically-conductive membranes (ECMs) for the electrosorptive separation of proteins (BSA and LYZ). *We highlight the mechanisms of protein electrosorption on ECMs and demonstrate their potential in adsorptive applications. *The study calculates interfacial force values and discusses the strength of electrostatic interactions using XDLVO calculations. * We validate the calculations through static adsorption experiments under various degrees of double-layer screening, i.e. various pH and ionic strengths. *The electrosorption of both proteins is reversible and closely linked to the surface charge. *Separation factors can be modulated in favor of either protein (BSA or LYZ) by application of different electrical potentials.

Files

Institutions

Technische Universitat Munchen

Categories

Interface Science, Chromatography, Protein Purification, Adsorption of Protein, Membrane Technology

Licence