20250124_TUM_BioSE_DR

Published: 24 January 2025| Version 1 | DOI: 10.17632/vcccx3k7mv.1
Contributors:
Dennis Roecker,
,
,
,
,

Description

*A novel approach utilizing electrically-conductive membranes (ECMs) for the electrosorptive separation of proteins (BSA and LYZ). *We highlight the mechanisms of protein electrosorption on ECMs and demonstrate their potential in adsorptive applications. *The study calculates interfacial force values and discusses the strength of electrostatic interactions using XDLVO calculations. * We validate the calculations through static adsorption experiments under various degrees of double-layer screening, i.e. various pH and ionic strengths. *The electrosorption of both proteins is reversible and closely linked to the surface charge. *Separation factors can be modulated in favor of either protein (BSA or LYZ) by application of different electrical potentials.

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Institutions

  • Technische Universitat Munchen

Categories

Interface Science, Chromatography, Protein Purification, Adsorption of Protein, Membrane Technology

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