Raw data from the quantification of interneuron density in retrosplenial cortex layers of control (air-exposed) and ethanol (EtOH)-exposed mice at the indicated postnatal days

Published: 23 May 2022| Version 2 | DOI: 10.17632/vcd3b286cm.2
Contributor:
Carlos Valenzuela

Description

It has been previously shown that 40% of murine cortical interneurons are eliminated via apoptosis during the first two weeks of postnatal development . We studied whether this process is affected by alcohol exposure using a mouse model of third trimester alcohol exposure. We used transgenic mice that express the Venus fluorescent protein in GABAergic interneurons under the control of the vesicular GABA transporter promoter (VGAT-Venus mice). Mice were exposed to air (controls) or ethanol for 4 hr/day on postnatal days 4 to 9 using vapor inhalation chambers. Transcardial perfusions were performed under anesthesia at postnatal days 5, 7, 10 and 30. Cryostat-prepared floating sections were stained with the fluorescent DNA stain, 4’6-diamidino-2-phenylindole (DAPI). Two investigators (Megan Barber and Casey McDonald) independently quantified the density of Venus-positive GABAergic interneurons in layers I, II-IV and V of the retrosplenial cortex, which is part of the limbic memory system, and is sensitive to ethanol-induced apoptosis during the first postnatal week. Four randomly selected parasagittal brain slices (i.e., sections) per mouse were analyzed. Male and female mice are identified by different symbols. The tables shows the animal identification information at the different postnatal days. For example, the data from air-exposed postnatal day 5 pups is from 1 female and 1 male pup from litter V313, 1 male pup from litter V317, and 1 male pup from litter V320. When possible, we tested pups from the same litter at different ages (e.g., V313 air-exposed pups were tested at postnatal days 5, 7, 10 and 27). The excel file included here has several tabs (sheets) as follows: 1. Raw data collected by investigator Megan Barber 2. Average interneuron counts and area in micrometer square from Megan Barber data 3. Interneuron density (per micrometer square) from Megan Barber data 4. Raw data collected by investigator Casey McDonald 5. Average interneuron counts and area in micrometer square from Casey McDonald data 6. Interneuron density (per micrometer square) from Casey McDonald data 7. Interneuron density (per millimeter square) from the average of Megan Barber and Casey McDonald data

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Institutions

University of New Mexico Health Sciences Center

Categories

Developmental Neuroscience, Development of the Cerebral Cortex, Fetal Alcohol Spectrum Disorder

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