DATASET: Bacteroides vesicles promote functional alterations in the gut microbiota composition

Published: 4 September 2024| Version 1 | DOI: 10.17632/vfprtkk4cm.1
Contributors:
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, Artemiy S,
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, Elizaveta Vorobeva, Maria Markelova, Evgenii Olekhnovich, Maxim Morozov, Polina Zoruk, Daria Boldyreva, Anna Vanyushkina, Andrei Chaplin, Tatiana Grigoryeva,
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Description

Inflammatory bowel diseases are characterized by chronic intestinal inflammation and alterations in the gut microbiota composition. Bacteroides fragilis, which secretes outer membrane vesicles (OMVs) with polysaccharide A (PSA), can moderate the inflammatory response and possibly alter the microbiota composition. In this study, we created a murine model of chronic DSS-induced intestinal colitis and treated it with Bacteroides fragilis OMVs. We monitored the efficiency of OMV therapy by determining the disease activity index (DAI) and performing histological examination (HE) of the intestine before and after vesicle exposure. We also analyzed the microbiota composition using 16S rRNA gene sequencing. Finally, we evaluated the volatile compound composition in the animals’ stools by HS-GC/MS to assess the functional activity of the microbiota. We observed more effective intestinal repair after OMV treatment according to the DAI and HE. A metabolomic study also revealed changes in the functional activity of the microbiota, with a predominance of phenol and pentanoic acid in the control group compared to the group treated with DSS (DSS) and the group treated with OMVs (DSS OMVs). We also observed a positive correlation of these metabolites with Saccharibacteria and Acetivibrio in the control group, whereas in the DSS group, there was a negative correlation of phenol and pentanoic acid with Lactococcus and Romboutsia. According to the metabolome and sequencing data, the microbiota composition of the DSS-treated OMV group was intermediate between that of the control and DSS groups. OMVs not only have an anti-inflammatory effect but also contribute to the recovery of the microbiota composition.

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Institutions

Institut obsej genetiki imeni N I Vavilova RAN, Kazanskij federal'nyj universitet Institut fundamental'noj mediciny i biologii, Federal'nyj naucno-kliniceskij centr fiziko-himiceskoj mediciny, Rossijskij nacional'nyj issledovatel'skij medicinskij universitet imeni N I Pirogova, Federal'nyj medicinskij issledovatel'skij centr psihiatrii i narkologii imeni V.P.Serbskogo

Categories

Vesicle, Metabolomics, Mass Spectrum, Bacteroides

Funding

Russian Science Foundation

21-75-10172

Licence