Astrocyte-derived extracellular vesicles enhance the electrophysiological function and cell survival of human cortical neurons in vitro

Published: 22 Jan 2020 | Version 1 | DOI: 10.17632/vg957ts286.1
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Description of this data

The data uploaded here are for the manuscript titled 'Astrocyte-derived extracellular vesicles enhance the electrophysiological function and cell survival of human cortical neurons in vitro '

Experiment data files

  • Apoptosis-FACS analysis
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  • Beta-Gal_Senescence assay
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    • 20190711-beta-gal test_@day4 (24 hrs after 3days diff)
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    • 20190717-beta-gal test_various timepoints with exosome
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  • Immunocytochemistry images
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    • 20190213
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    • 20190215
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    • 20190307
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      • Astrocyte and Schwann cells_heterogeneity test
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        • hAstrocyte
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        • hSchwann cell
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      • Astrocytes on 5%_FL_Nafion
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      • Astrocytes on 5%_NafionP
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    • 20190408
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      • NGN2_Neurons (d4)_5%Nafion alignment_16 dfferent patterns
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        • 16
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        • 5
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      • NGN2_Neurons (d4)_CUX1 expression
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    • 20190505
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      • 20190506_Exoglow_test_1
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    • 20190702
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    • 20190703_CUX1 expression_60x
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    • 20190703_Exosome_uptake_60x
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      • 1_100 (exosome to cell ratio)
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      • 1_25 (exosome to cell ratio)
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      • 1_250 (exosome to cell ratio)
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    • 20190717_hAC exosome_intensity comparison
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    • 20190928_low density axon length
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  • Nanosight particle analysis
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    • 12hrs_incubation
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    • 24hrs_incubation
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    • 36hrs_incubation
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    • 48hrs_incubation
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    • 84hrs_incubationi
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    • hAC_made by Changho_on_190208
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    • Incubation time vs. Exosome density
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      • 12hrs_incubation
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      • 24hrs_incubation
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      • 36hrs_incubation
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      • 48hrs_incubation
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      • 84hrs_incubationi
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  • Patch clamp analysis
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    • 20190305_0306-NGN2 test1 D28
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      • 20190305_0306-NGN2 test1 D28
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        • 20190305 - NGN2 test 1 D28 (raw data)
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        • AP trace
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        • APD_Up and Down_Slope
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        • Raw data
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      • 20190606 - NGN2 test 1 D14
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      • 20190620_0621-NGN2 test2 D28
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      • 20190912_NGN2 test 3 D28_consolidated
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        • Raw data
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          • 20190620
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          • 20190912
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    • 20190606 - NGN2 test 1 D14
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    • 20190620_0621-NGN2 test2 D28
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    • 20190912_NGN2 test 3 D28_consolidated
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      • Raw data
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        • 20190620
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        • 20190912
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Latest version

  • Version 1

    2020-01-22

    Published: 2020-01-22

    DOI: 10.17632/vg957ts286.1

    Cite this dataset

    Kim, Deok-Ho; Chun, Changho; Smith, Alec (2020), “Astrocyte-derived extracellular vesicles enhance the electrophysiological function and cell survival of human cortical neurons in vitro ”, Mendeley Data, v1 http://dx.doi.org/10.17632/vg957ts286.1

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Categories

Flow Cytometry, Patch Clamp Technique, Image Analysis

Licence

CC BY 4.0 Learn more

The files associated with this dataset are licensed under a Creative Commons Attribution 4.0 International licence.

What does this mean?
You can share, copy and modify this dataset so long as you give appropriate credit, provide a link to the CC BY license, and indicate if changes were made, but you may not do so in a way that suggests the rights holder has endorsed you or your use of the dataset. Note that further permission may be required for any content within the dataset that is identified as belonging to a third party.

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